and transmitted securely. We determined the sensitivity of the influenza virus strain H1N1 (A/PR8/34) and the formerly pandemic H3N2 (A/Aichi/2/68) to carrageenans of subtypes iota and kappa by plaque reduction assays in MDCK cells .The results are summarized in Table 1.The two carrageenan types inhibited plaque formation of both viruses . Viral load - influenza virus titres in lung homogenates, nasal washes or BAL. Would you like email updates of new search results? Attenuated negative strand viruses with altered interferon antagonist activity for use as vaccines and pharmaceuticalsAttenuated negative strand viruses with altered interferon antagonist activity for use as vaccines and pharmaceuticals .. .. . Max A. Lauffer, W. M. Stanley; BIOPHYSICAL PROPERTIES OF PREPARATIONS OF PR8 INFLUENZA VIRUS . To identify Ab-dependent properties and serum components that contributed to enhancement of Ab activity, we screened a large panel of HA-specific MAbs for hemagglutination inhibition (HI) in the presence of noninactivated serum from naive mice (NMS). Careers. The (re)emergence of new influenza viruses or SARS-CoV-2 variants, confirms that we are in front of recurrent and unpredictable risk of epidemics and pandemic. MeClelland, L., Canadian Public Health Journal 32: 530 (1941). Influenza A_N1_antiviral-response_278(1)_E258Q_Resistance to Oseltamivir: 278Q: No: Introduction of Glu258Gln naturally occurring substitution in the A/Vietnam/1203/2004 backbone decreased oseltamivir sensitivity using plaque reduction assay in MDCK cells. The biophysical properties of several preparations of PR8 influenza virus have been studied. Bookshelf Peer-reviewed Research Article Antibodies with 'Original Antigenic Sin' Properties Are Valuable Components of Secondary Immune Responses to Influenza Viruses Susanne L. Linderman, AffiliationsDepartment of Microbiology, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, Pennsylvania, United States of America, Clinical scores following infection with PR8 (H1N1 influenza). Phylogenetic analysis revealed that the genome sequences of . 75N93019C00076, awarded to the University of Chicago. 1989 Apr;63(4):1704-14. doi: 10.1128/JVI.63.4.1704-1714.1989. Cellular infiltrate and cytokines in BAL or lung homogenate Gross pathology scores Validation Data Figure 1. Influenza vaccine manufacturers require antigenically relevant vaccine viruses that have good manufacturing properties and are safe to use. [Identification of the antigenic components of the host cell in isolated glycoproteins of the influenza virus]. After purification by density gradient procedures, the virus preparations were tested for the presence of host antigens with . The polymer exhibited virus-inhibiting properties with an IC 50 = 1 g/mL and a selectivity index of 302. Morphologial and structural analysis of influenza virus PR8 strain at pH 5.5 In this condition, most virions have no apparent structural changes ( Fig. J Exp Med. Click here to visit your profile and update your email address and/or resend the verification message. PR8 influenza virus Nasal immunization 1. Would you like email updates of new search results? Electron micrographs showed slightly irregular, circular particles with an average diameter of 115 mmicro and a standard deviation of the distribution of diameters of 15 per cent. FOIA SARS-CoV-2 Genome Assembly and Annotation. CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): During the past decade several studies on the physical properties of various strains of influenza virus have been reported. This corresponds to a distribution of diameters with a standard deviation of 4 per cent of the mean. HHS Vulnerability Disclosure, Help 1944 Dec 1;80(6):521-9. doi: 10.1084/jem.80.6.521. This project has been funded in whole or in part with Federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under Contract No. PubMed:17296744: NA: Influenza A_N1_antiviral-response_295(1)_N275S_Resistance to . Please contact us if you have any questions or issues. J Virol. Lung-adapted PR8 virus was used as a control. 1944 Feb 1;79(2):197-203 Addresses across the entire subnet were used to download content in bulk, in violation of the terms of the PMC Copyright Notice. Virus Res. Use of PMC is free, but must comply with the terms of the Copyright Notice on the PMC site. More than a million books are available now via BitTorrent. National Library of Medicine During the course of searching for antiviral agents from Chinese medicinal herbs, we found that the supercritical fluid extract (SFE) of Centipeda minima possessed good in vitro antiviral activity . First, the density of the spike proteins has become blurred in some localized areas of the envelope. PMC This product is whole-genome sequenced and has applications in influenza research. 1944 Mar 1;79(3):285-90 Disclaimer, National Library of Medicine For example, infection of mice with the pathogenic PR8 strain of influenza virus (A/Puerto Rico/8/1934 [H1N1]) at a sublethal dose results in spread of the virus to the heart and replication to low levels ( 9, 10 ), but the resulting myocarditis is mild and resolves quickly as the virus is cleared ( 9 ). The immunogenicity in mice of virus that had been exposed to A. niger extract appeared to be very broad, since vaccination of mice with such material gave protection against challenge with strains of influenza A virus representing all major serotypes as well as one type B influenza virus. BPL reacts readily with nucleophiles, resulting in alkylated and acylated products. The specific volume was determined with a pycnometer to be 0.79. For requests to be unblocked, you must include all of the information in the box above in your message. THE PREPARATION OF HIGHLY PURIFIED PR8 INFLUENZA VIRUS FROM INFECTED MOUSE LUNGS. official website and that any information you provide is encrypted The https:// ensures that you are connecting to the The .gov means its official. government site. Your email has not been verified. Try another strain of virus in parallel, but not a recent H3N2 or pdm2009 for. strain of human influenza and the Shope strain of swine influenza virus. My Research and Language Selection Sign into My Research Create My Research Account English; Help and support. Identification of morphological differences between avian influenza A viruses grown in chicken and duck cells. Careers. 1944 Mar 1;79(3):267-83. doi: 10.1084/jem.79.3.267. Epub 2015 Jan 19. J Exp Med. The new BV-BRC system is built on the PATRIC Bacterial BRC system and incorporates data and tools from IRD and ViPR. Careers. Al-Mubarak F, Daly J, Christie D, Fountain D, Dunham SP. 5. INFLUENCE OF pH AND OF CERTAIN OTHER CONDITIONS ON THE STABILITY OF THE INFECTIVITY AND RED CELL AGGLUTINATING ACTIVITY OF INFLUENZA VIRUS. The specific volume was determined with a pycnometer to be 0.79. The migration of the data, tools, and services from the legacy IRD and ViPR applications to BV-BRC is now complete. Furthermore, the Lee virus was much less stable in dilute solution in phosphate buffer, even under optimum conditions of pH, than either the PR8 or swine strains. Type I interferon regulates proteolysis by macrophages to prevent immunopathology following viral infection Federal government websites often end in .gov or .mil. J Exp Med. However, reassortants with H5-derived HA with a polybasic cleavage site are potentially hazardous for animal health. The variation of sedimentation rate with concentration was shown to be due entirely to the variation of solution viscosity with concentration. The present disclosure provides small hairpin nucleic acid molecules capable of stimulating interferon production. Mortality rates typically increase further during . the h5 seed virus (re-8) contains the ha and na genes from the clade 2.3.4.4 virus a/chicken/guizhou/4/2013 (h5n1) and its six internal genes from the high-growth a/puerto rico/8/1934 (h1n1) (pr8) virus and has been used in china since 2015 [ 7, 10 ], and the h7 seed virus (h7-re1) is a reassortant bearing the ha and na genes of the h7n9 low Please refer to this Quick Start Guide to learn how to start using BV-BRC. J Exp Med. Journal Archive Collections Pearls Reviews Opinions Editorials Find and Read Articles Publish Submissions Getting Started Submission Guidelines Figures Tables Data Sharing Support Supporting Information LaTeX Other Article Types Preprints Revising Your Manuscript Submit Now Policies Best Practices Research. The CPSF inhibitory properties of NS1 appear to be dispensable for some influenza virus strains . Our non-RG Cambridge lineage PR8 is a much worse plaque former than the RG clone we got from Ron Fouchier. Together, these data demonstrate that SERINC5 . If you are transitioning from PATRIC or IRD/ViPR, please refer to the Quick Start Guide to learn how to get started with BV-BRC. The IP address used for your Internet connection is part of a subnet that has been blocked from access to PubMed Central. Dear IRD and ViPR users, welcome to BV-BRC. Clipboard, Search History, and several other advanced features are temporarily unavailable. we address these issues by examining the differential properties of HA head and stalk-binding . 8600 Rockville Pike Electron micrographs showed slightly irregular, circular particles with an average diameter of 115 mmicro and a standard deviation of the distribution of diameters of 15 per cent. This drug-induced variant (DIV) has the following properties: 1. PRECIPITIN REACTIONS OF HIGHLY PURIFIED INFLUENZA VIRUSES AND RELATED MATERIALS. Clipboard, Search History, and several other advanced features are temporarily unavailable. The virus was shown not to lose infectivity very rapidly in sucrose solutions. -, J Exp Med. The PR8 strain of influenza A virus was grown in canine kidney cell cultures and in chick and duck embryos. Conformational changes in myosin and heavy meromyosin from chicken gizzard associated with phosphorylation. The site is secure. The intrinsic viscosities, calculated on the basis of concentrations expressed as grams per cubic centimeter, of several highly purified preparations were determined to be between 11.3 and 16.5. Body weight loss in mice infected with 50 and 500 PFU of PR8 virus was almost 4% and 13% at 3 dpi, and 17% and 25% at 6 dpi, respectively. combination vaccinescombination vaccines .. .. ELECTROPHORETIC STUDIES ON PR8 INFLUENZA VIRUS. The average particle diameter of the anhydrous PR8 virus particle was calculated from sedimentation and specific volume data to be about 80 m. The density in the absence of sucrose was estimated to be about 1.1. Antiviral properties of the NSAID drug naproxen Targeting the nucleoprotein of SARS-CoV-2 Coronavirus . HHS Vulnerability Disclosure, Help . These types of effects would disrupt the ordered lipid rafts ( 32, 33) and leaflet asymmetry ( 34) in influenza viral envelopes. An official website of the United States government. . The variation of sedimentation rate with concentration was shown to be due entirely to the variation of solution viscosity with concentration. The invention relates to a nucleic acid construct encoding at least three RNA molecules comprising a double-stranded region, wherein the RNA molecules reduce influenza A virus replication in an animal cell and/or reduce production of infectious influenza A virus particles in an animal cell and/or reduce the expression of an influenza A virus polypeptide in an influenza A virus infected animal . sharing sensitive information, make sure youre on a federal Preparation of inactivated influenza vaccines is conventionally achieved by exposure of cell- or egg-derived live virus to beta-propiolactone (BPL) or formaldehyde (FA). The following materials are designed to aid IRD and ViPR users in using BV-BRC. From the Department of Animal and Plant Pathology of The Rockefeller Institute for Medical Research, Princeton, New Jersey. J Exp Med. An official website of the United States government. J Gen Virol. . Mice were inoculated intranasally with non-lung-adapted influenza viruses of subtype H1N1pdm09. Bethesda, MD 20894, Web Policies Electron micrographs showed slightly irregular, circular particles with an average diameter of 115 m and a standard deviation of the distribution of diameters of 15 per cent. Viral antigens and impurities in influenza vaccines. It was antigenically different from the influenza A (PR8) virus, but had the same properties in terms of culture in eggs. doi: https://doi.org/10.1084/jem.80.6.531. An official website of the United States government. LAUFFER, M.A., Biophysical properties of preparations of PR8 influenza virus, JOURNAL OF EXPERIMENTAL MEDICINE 80: 531 (1944). Europe PMC is an archive of life sciences journal literature. Evidence is reported which suggests that cellular actin is an integral component of highly purified PR8 virions, though probably unrelated to host antigen. The sedimentation rate was found to vary inversely with the concentration of virus. Evaluation by immunoelectrophoretic methods. They were monitored closely for body weight loss, mortality outcomes and gross pathology for 14 days following inoculation, as well as viral replication in lung tissue. Brief exposure of PR8 influenza virus at pH 4.5 to extracts of Aspergillus niger abolished the red cell . government site. Influenza virus infection potently induces expression of both pro- and anti-inflammatory genes in vivo that contribute to the inflammatory response . N.Y.) and is referred to as PR8. Influenza virus fusion events can be illustrated by a shift in the fluorescence color of virus particles. Introduction Although aluminum in virtually all currently adjuvant human vaccines is used to help increase the humoral or cellular immune responses to an antigen, it usually induces a Th2 antibody dominated response. Data are presented as mean clinical scores SEM (n=10). P09 (A/H1N1pdm09) and H3N2 (A/Texas/50/2012) in the SERINC5-overexpressing A549 cells. In certain embodiments, the molecule comprises at least one 5-triphosphate and/or at least one 5-diphosphate. The https:// ensures that you are connecting to the Fig. J Exp Med. sharing sensitive information, make sure youre on a federal government site. Prophylactic intranasal administration of the polymer in a murine model of influenza pneumonia completely prevented the virus-induced death of animals, whereas the level of mortality in the placebo group was 90%. In developing pandemic vaccine viruses, reverse genetics has been employed as a rational approach that can also be used effectively to attenuate the highly virulent H5N1 virus and at the same time place the H5 HA and N1 NA on a background of PR8, a virus . Upload and analyze your data in the private workspace. ; Contact Us Have a question, idea, or some feedback? Sedimentation studies in sucrose solutions of varying densities showed a non-linear dependence of sedimentation rate upon solvent density, indicating that the density in solution increases with increasing sugar concentration. After purification by density gradient procedures, the virus preparations were tested for the presence of host antigens with antisera to appropriate host materials. MeSH To examine that our model can recognize influenza CPE induced by different influenza virus strains, we collected about 120 image of MDCK cells infected by pandemic H1N1/09, H3N2, and influenza B at 16 . Interleukin-7R in the regulation of lymphoid anti-viral responses and homeostasis . Proposed mechanism of influenza virus inactivation by N, N -dodecyl,methyl-PEI coatings. All web traffic from the IRD and ViPR resources is also being forwarded to the BV-BRC home page. The virus was shown not to lose infectivity very rapidly in sucrose solutions. official website and that any information you provide is encrypted La Biblioteca Virtual en Salud es una coleccin de fuentes de informacin cientfica y tcnica en salud organizada y almacenada en formato electrnico en la Regin de Amrica Latina y el Caribe, accesible de forma universal en Internet de modo compatible con las bases internacionales. We have used mAb 1A8 (anti-Ly6G) to specifically deplete LyG6high neutrophils and induce neutropenia in mice infected with virus strains known to differ in virulence. The PR8 strain of influenza A virus was grown in canine kidney cell cultures and in chick and duck embryos. The .gov means its official. The viscosity of the virus preparations can be explained as being due in great part to the presence of the slower sedimenting contaminating components which possess a very high intrinsic viscosity. Sixteen hemagglutinin subtypes are associated with wild waterfowl hosts; some subtypes are isolated infrequently, one of which is H12 IAV. We want to hear from you. The density in the absence of sucrose was estimated to be about 1.1. Support Center Find answers to questions about products, access, use, setup, and administration. }, author={J. Bronwyn Bateman and A. Zellner and M. Stuart Davis and P A McCAFFREY}, journal={Archives of . If you have questions or need assistance, please contact us at help@bv-brc.org. Sedimentation studies in sucrose solutions of varying densities showed a non-linear dependence of sedimentation rate upon solvent density, indicating that the density in solution increases with increasing sugar concentration. In this study, we detected three H12 IAVs from Anascrecca and Anas formosa in Poyang Lake, China, in 2018, one of which was isolated. It is estimated that such hydrated virus particles are about 100 mmicro in diameter. J Exp Med. ( A) An influenza viral particle diffuses to the polycation-coated surface from solution and ( B) adheres to it. These host antigens appeared not to be closely associated with the hemagglutinin or neuraminidase spikes, since virus from which essentially all of these structures were removed formed substantial precipitates with antiserum to host material. Before 1944 Dec 1;80(6):549-59 [26] This virus was named Influenza B and the inactivated. PLOS Pathogens publishes Open Access research and commentary that significantly advance the understanding of pathogens and how they interact with host organisms.. Get Started Apigenin suppresses influenza A virus-induced RIG-I activation and viral replication Flavonoid apigenin is low toxicity and few favorable bioactivities, including anti-tumor properties, antioxidant, anti-inflammatory activity and antiviral. J Exp Med 1 December 1944; 80 (6): 531548. Both approaches utilize a high growth virus, generally A/Puerto Rico/8/1934 (PR8), as . 2015 Mar 2;199:9-19. doi: 10.1016/j.virusres.2015.01.005. The current seasonal and pre-pandemic vaccine viruses are generated either by classical reassortment or reverse genetics. A combination of high-yield mutations from these screens led to a PR8 backbone that improved the titres of H1N1, H3N2, H5N1 and H7N9 vaccine viruses in African green monkey kidney and. cH5/1 PR8 N3 virus or Cal/09 N3 virus (100 PFU) was . . Viral data and analysis tools and services from IRD/ViPR have now been integrated into the resource. FOIA The biophysical properties of several preparations of PR8 influenza virus have been studied. By continuing to use our website, you are agreeing to, CENTRIFUGATION AND ULTRAFILTRATION STUDIES ON ALLANTOIC FLUID PREPARATIONS OF INFLUENZA VIRUS, THE PREPARATION OF HIGHLY PURIFIED PR8 INFLUENZA VIRUS FROM INFECTED MOUSE LUNGS, ELECTROPHORETIC STUDIES ON PR8 INFLUENZA VIRUS. Federal government websites often end in .gov or .mil. For additional information, or to request that your IP address be unblocked, please send an email to PMC. official website and that any information you provide is encrypted The site is secure. Lung virus titers in the lungs of PR8 infected mice treated with Andro, CL-385319 or both (10 mg/kg). A SEDIMENTABLE COMPONENT OF ALLANTOIC FLUID AND ITS RELATIONSHIP TO INFLUENZA VIRUSES. 1944 Aug 1;80(2):83-100. doi: 10.1084/jem.80.2.83. Find diseases associated with this biological target and compounds tested against it in bioassay experiments. Influenza virus . Product category Viruses Product type Effects of cell differentiation on replication of A/WS/33, WSN, and A/PR/8/34 influenza viruses in mouse brain cell cultures: biological and immunological characterization of products. Although both PR8 and CA04 belong to H1N1 IAV, they have distinct origins ( Califano et al., 2018 ). Unable to load your collection due to an error, Unable to load your delegates due to an error. Disclaimer, National Library of Medicine Accessibility DOI: 10.1016/0003-9861(56)90441-6 Corpus ID: 41895502; The electrophoretic properties of red blood cells after reaction with influenza virus hemagglutinin. These two strains were used to infect human 293T cells as a representative human cell line and A549 cells (human lung epithelial cell line) as a more physiologically relevant cell line ( Geiss et al., 2002; Temperton et al., 2007 ). Summary Certain biological properties of an influenza A virus variant, obtained in vivo from the lung tissue of mice infected with A-PR 8 influenza virus and treated with Xenalamine, have been studied. By contrast with SP-D, human SP-A also has considerable HA inhibitory activity for PR8 (Hartshorn, manuscript submitted). Accessibility 532 BIOPHYSICAL PROPERTIES OF PR8 INFLUENZA VIRUS this study they observed that the infectious principles of the A and B strains have filtration end-points of 163 and 180 m#, respectively, indicating that the infectious particles have diameters within the range 80 to 135 m/~ (8). The .gov means its official. Mice were also treated with mAb RB6-8C5 (anti-Ly6C/G or anti-Gr-1), a mAb widely used to investigate the role of . All PR8 preparations were shown to have serologically detectable host components. To update the vaccine strains in the antigen bank, we used reverse genetics technology to develop two vaccine candidates against avian influenza strains belonging to clades 2.3.2.1d and 2.3.4.4h, and then evaluated their immunogenicity and . Iota-carrageenan inhibits influenza virus-induced plaque formation on MDCK cells. MeSH We used PR8 virus as our model virus to address this question. The biophysical properties of several preparations of PR8 influenza virus have been studied. Influenza A virus PB1-F2, encoding a multi-functional protein, is regarded as a virulent gene. Seasonal influenza infection is associated with 200,000-500,000 deaths each year, particularly in young children, immunocompromised patients and the elderly. HHS Vulnerability Disclosure, Help Abstract Highly purified preparations of PR8 influenza virus were obtained from perfused, infected mouse lungs by a combination of methods involving adsorption of the virus on and elution from chicken red cells and differential centrifugation. One of the important lessons learned from the 2009 H1N1 pandemic is that a high yield influenza vaccine virus is essential for efficient and timely production of pandemic vaccines in eggs. The spreading of the boundary during sedimentation was shown to be represented by a standard deviation of the sedimentation rate equal to 8 per cent of the mean. Bethesda, MD 20894, Web Policies Search for other works by this author on: Copyright, 1944, by The Rockefeller Institute for Medical Research New York, This site uses cookies. 8600 Rockville Pike Bethesda, MD 20894, Web Policies Modification du fond gntique PR8 pour l'optimisation qualitative et . Become blurred in some localized areas of the Copyright Notice also evaluated general condition, rate The NSAID drug naproxen Targeting the nucleoprotein of SARS-CoV-2 Coronavirus from infected MOUSE lungs: //www.fludb.org/brc/fluStrainDetails.spg? strainName=A/PR8/1934 decorator=influenza Or lung homogenate Gross pathology scores Validation data Figure 1 being forwarded to the inflammatory. ( A/Texas/50/2012 ) in the absence of sucrose was estimated to be about 1.1 PR8 virus as model! Subnet that has been blocked from access to PubMed Central or by sugars expected to block collectin.. 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Biology < /a > your email has not been verified of both pro- and anti-inflammatory genes in vivo contribute! The inhibitory activity for PR8 ( Hartshorn, manuscript submitted ) Search History, and several other advanced are.
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